The dark side of centromeres: types, causes and consequences of structural abnormalities implicating centromeric DNA

Centromeres are the chromosomal domains required to ensure faithful transmission of the genome during cell division. They have a central role in preventing aneuploidy, by orchestrating the assembly of several components required for chromosome separation. However, centromeres also adopt a complex structure that makes them susceptible to being sites of chromosome rearrangements. Therefore, preservation of centromere integrity is a difficult, but important task for the cell. In this review, we discuss how centromeres could potentially be a source of genome instability and how centromere aberrations and rearrangements are linked with human diseases such as cancer

Fluid dynamic aspects of jet noise generation

Jet engine noise generation and noise propagation was investigated by studying supersonic nozzle flow of various nozzle configurations in an experimental test facility. The experimental facility was constructed to provide a coaxial axisymmetric jet flow of unheated air. In the test setup, an inner primary flow exhausted from a 7 in. exit diameter convergent--divergent nozzle at Mach 2, while a secondary flow had a 10 in. outside diameter and was sonic at the exit. The large dimensions of the jets permitted probes to be placed inside the jet core without significantly disturbing the flow. Static pressure fluctuations were measured for the flows. The nozzles were designed for shock free (balanced) flow at Mach 2. Data processing techniques and experimental procedures were developed in order to study induced disturbances at the edge of the supersonic flows, and the propagation of those disturbances throughout the flows. Equipment used (specifications are given) to record acoustic levels (far field noise) is described. Results and conclusions are presented and discussed. Diagrams of the jet flow fields are included along with photographs of the test stand

A linear Stark shift in dressed atoms as a signal to measure a nuclear anapole moment with a cold atom fountain or interferometer

We demonstrate theoretically the existence of a linear dc Stark shift of the individual substates of an alkali atom in its ground state, dressed by a circularly polarized laser field. It arises from the electroweak nuclear anapole moment violating P but not T. It is characterized by the pseudoscalar equal to the mixed product formed with the photon angular momentum and static electric and magnetic fields. We derive the relevant left-right asymmetry with its complete signature in a field configuration selected for a precision measurement with cold atom beams. The 3,3 to 4,3 Cs hyperfine-transition frequency shift amounts to 7 $\mu$Hz for a laser power of about 1 kW at 877 nm, E=100 kV/cm and B larger than 0.5 G.Comment: Article, 4 pages, 2 figure

P14ARF: The absence that makes the difference

P14ARF is a tumor suppressor encoded by the CDKN2a locus that is frequently inactivated in human tumors. P14ARF protein quenches oncogene stimuli by inhibiting cell cycle progression and inducing apoptosis. P14ARF functions can be played through interactions with several proteins. However, the majority of its activities are notoriously mediated by the p53 protein. Interestingly, recent studies suggest a new role of p14ARF in the maintenance of chromosome stability. Here, we deepened this new facet of p14ARF which we believe is relevant to its tumor suppressive role in the cell. To this aim, we generated a monoclonal HCT116 cell line expressing the p14ARF cDNA cloned in the piggyback vector and then induced aneuploidy by treating HCT116 cells with the CENP‐E inhibitor GSK923295. P14ARF ectopic re‐expression restored the near‐diploid phenotype of HCT116 cells, confirming that p14ARF counteracts aneuploid cell generation/proliferation

Magnetization dynamics in the single-molecule magnet Fe8 under pulsed microwave irradiation

We present measurements on the single molecule magnet Fe8 in the presence of pulsed microwave radiation at 118 GHz. The spin dynamics is studied via time resolved magnetization experiments using a Hall probe magnetometer. We investigate the relaxation behavior of magnetization after the microwave pulse. The analysis of the experimental data is performed in terms of different contributions to the magnetization after-pulse relaxation. We find that the phonon bottleneck with a characteristic relaxation time of 10 to 100 ms strongly affects the magnetization dynamics. In addition, the spatial effect of spin diffusion is evidenced by using samples of different sizes and different ways of the sample's irradiation with microwaves.Comment: 14 pages, 12 figure

Surface doping in T6/ PDI-8CN2 Heterostructures investigated by transport and photoemission measurements

In this paper, we discuss the surface doping in sexithiophene (T6) organic field-effect transistors by PDI-8CN2. We show that an accumulation heterojunction is formed at the interface between the organic semiconductors and that the consequent band bending in T6 caused by PDI-8CN2 deposition can be addressed as the cause of the surface doping in T6 transistors. Several evidences of this phenomenon have been furnished both by electrical transport and photoemission measurements, namely the increase in the conductivity, the shift of the threshold voltage and the shift of the T6 HOMO peak towards higher binding energies.Comment: 5 pages, 5 figure

Very low bias stress in n-type organic single crystal transistors

Bias stress effects in n-channel organic field-effect transistors (OFETs) are investigated using PDIF-CN2 single-crystal devices with Cytop gate dielectric, both under vacuum and in ambient. We find that the amount of bias stress is very small as compared to all (p-channel) OFETs reported in the literature. Stressing the PDIF-CN2 devices by applying 80 V to the gate for up to a week results in a decrease of the source drain current of only ~1% under vacuum and ~10% in air. This remarkable stability of the devices leads to characteristic time constants, extracted by fitting the data with a stretched exponential - that are \tau ~ 2\cdot10^9 s in air and \tau ~ 5\cdot10^9 s in vacuum - approximately two orders of magnitude larger than the best values reported previously for p-channel OFETs.Comment: Submitted to Applied Physics Letters; 14 pages, 3 figure

CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly

Human centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive alphoid DNA sequences. By inducing rapid, complete degradation of endogenous CENP-A, we now demonstrate that once the first steps of centromere assembly have been completed in G1/S, continued CENP-A binding is not required for maintaining kinetochore attachment to centromeres or for centromere function in the next mitosis. Degradation of CENP-A prior to kinetochore assembly is found to block deposition of CENP-C and CENP-N, but not CENP-T, thereby producing defective kinetochores and failure of chromosome segregation. Without the continuing presence of CENP-A, CENP-B binding to alphoid DNA sequences becomes essential to preserve anchoring of CENP-C and the kinetochore to each centromere. Thus, there is a reciprocal interdependency of CENP-A chromatin and the underlying repetitive centromere DNA sequences bound by CENP-B in the maintenance of human chromosome segregation